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The immature platelet fraction: An assessment of its application to a routine clinical laboratory

Australian Journal of Medical Science
Volume 33 Issue 2 (Jun 2012)

Abstract: The Sysmex XE-2100 and XE-5000 automated haematology analyzers (Sysmex, Kobe, Japan) offer an optional parameter called the Immature Platelet Fraction (IPF), which can be quickly and easily performed as a specifically ordered or reflex test for the investigation of thrombocytopenia. The purpose of this study was to assess its value for everyday use in a routine clinical laboratory servicing community collection centres, hospitals and specialist clinics. The reference interval was established on 114 normal patients as 0.7-5.5% with a mean of 2.0%, median of 1.6%, and standard deviation of 1.2%. Stability studies performed showed that specimens should be analyzed within 8h of collection and stored at room temperature, with no significant difference between storage at room temperature or 4 degreesC within the 8h time frame. Reproducibility on patient samples was dependent on the IPF% range, with the highest coefficient of variation (CV) found for the lowest numerical values. The average CV was 19%. A review of 4060 routine samples showed that when tested within 8h of collection, 1.7% of patients with a normal platelet count had a raised IPF%, compared to 30.9% in the thrombocytopenic population. In patients undergoing bone marrow biopsy, the highest IPF% values were found in those with normal to increased megakaryocyte numbers or with dysplastic morphology. As a direct measure of peripheral platelet destruction, this study found it has suitable applications in a central haematology laboratory.

To cite this article: Sinclair, Leanne. The immature platelet fraction: An assessment of its application to a routine clinical laboratory [online]. Australian Journal of Medical Science, Vol. 33, No. 2, Jun 2012: 48-57. Availability: <http://search.informit.com.au/documentSummary;dn=548339320391320;res=IELHEA> ISSN: 1038-1643. [cited 24 Aug 16].

Personal Author: Sinclair, Leanne; Source: Australian Journal of Medical Science, Vol. 33, No. 2, Jun 2012: 48-57 Document Type: Journal Article ISSN: 1038-1643 Subject: Diagnosis, Laboratory; Thrombocytopenia; Biopsy; Health risk assessment; Patients--Attitudes; Peer Reviewed: Yes Affiliation: (1) Wesley Hospital Laboratory, Sullivan Nicolaides Pathology, Wesley Private Hospital, Suite 54, Sandford Jackson Building, 30 Chasely Street, Auchenflower QLD 4066, Brisbane, Queensland, email: leanne_sinclair@snp.com.au

Database: Health Collection