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Comparison of the effectiveness of issr and ssr markers in determination of date palm ('Phoenix dactylifera' L.) Agronomic traits

Australian Journal of Crop Science
Volume 7 Issue 6 (May 2013)

Abstract: Date palm ('Phoenix dactylifera' L.) is extensively cultivated in the Middle East and in North Africa. Seven ISSR markers and five SSR loci were selected and used to evaluate genetic diversity in twenty-six Tunisian cultivars. ISSR primers amplified a total of 43 polymorphic DNA fragments. The average was 6.1 fragments per primer. The microsatellites examined in this study were highly polymorphic possessing a great number of alleles with an average of 7.2 alleles per locus. Principal component analyses based on Nei Genetic distances showed groups of cultivars with a common maturity period and a common fruit consistency. SSR markers discriminate the fruit characteristics subpopulations in a more convincing way than ISSR markers. The Mantel test emphasizes a significant correlation between genetic distance and fruit consistency. A significant difference was observed between soft and dry subpopulations using ISSR data and between semisoft and the other fruit consistency subpopulations using SSR data.

To cite this article: Hamza, Hammadi; Ben Abederrahim, Mohammed Ali; Elbekkay, Mokhtar and Ferchichi, Ali. Comparison of the effectiveness of issr and ssr markers in determination of date palm ('Phoenix dactylifera' L.) Agronomic traits [online]. Australian Journal of Crop Science, Vol. 7, No. 6, May 2013: 763-769. Availability: <http://search.informit.com.au/documentSummary;dn=365469654273211;res=IELHSS> ISSN: 1835-2693. [cited 09 Dec 16].

Personal Author: Hamza, Hammadi; Ben Abederrahim, Mohammed Ali; Elbekkay, Mokhtar; Ferchichi, Ali; Source: Australian Journal of Crop Science, Vol. 7, No. 6, May 2013: 763-769 Document Type: Journal Article ISSN: 1835-2693 Subject: Agronomy; Plant diversity; Plant varieties; Peer Reviewed: Yes Affiliation: (1) Arid and Oases Cropping Laboratory, Arid Area Institute, Medenine 4119, Tunisia
(2) Arid and Oases Cropping Laboratory, Arid Area Institute, Medenine 4119, Tunisia
(3) Arid and Oases Cropping Laboratory, Arid Area Institute, Medenine 4119, Tunisia
(4) Arid and Oases Cropping Laboratory, Arid Area Institute, Medenine 4119, Tunisia

Database: Humanities & Social Sciences Collection